Letter | Published:

Ultra-Violet Absorption in Living and Dead Cells

Nature volume 158, pages 663664 (09 November 1946) | Download Citation

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Abstract

AN ultra-violet microscope having an achromatic objective designed by Brumberg and Gershgorin1 (with an aperture of 0·5) has been used for photographing living tissue cultures. The latter were grown by the hanging drop method on a quartz cover-glass. The source of light used was a high-pressure quartz mercury lamp. All radiation except that of wavelengths 254–275 µµ were prevented from reaching the object by means of filters (bromine and chlorine vapours and Corning's Red Purple Corex A, No. 986 Glass). Focusing was performed under conditions of visible light (usually in a dark field) which completely prevented the ultra-violet rays from reaching the cells previous to their being photographed. The time of exposure was 20 sec.

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References

  1. 1.

    , Nature, 152, 357 (1943).

  2. 2.

    , Skand. Arch. Physiol., Suppl. No. 8 (1936).

  3. 3.

    , and , C.R. Acad. Sci. U.R.S.S., in the press.

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Author information

Affiliations

  1. Luminescence Laboratory of the State Optical Institute, and Cancer Research Laboratory of the Central Roentgenological, Radiological and Cancer Institute, Leningrad.

    • E. M. BRUMBERG
    •  & L. TH. LARINOW

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DOI

https://doi.org/10.1038/158663c0

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