Isolation of Pregnane-3(a)-ol-20-one from the Hydrolysis Products of 'Sodium Pregnanediol Glucuronidate'


Astwood and Jones1, and Talbot et al.2, have shown that not more than about 70 per cent of the theoretical amount of pregnanediol can be obtained from sodium pregnanediol glucuronidate after hydrolysis with hydrochloric acid. Using a colorimetric method of determining pregnanediol involving the development of a yellow colour on treatment with concentrated sulphuric acid, which is essentially similar to the method employed by Talbot et al., we have confirmed this finding. In the belief that the low yield of pregnanediol obtainable from the glucuronide was due to partial destruction in side-reactions during the hydrolysis, we decided to attempt the isolation and identification of the destruction products.

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  1. 1

    Astwood, E. B., and Jones, G. E. S., J. Biol. Chem., 137, 397 (1941).

  2. 2

    Talbot, N. B., Berman, R. A., Maclachlan, E. A., and Wolfe, J. K., J. Clin. Endocrin., 1, 668 (1941).

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MARRIAN, G., GOUGH, N. Isolation of Pregnane-3(a)-ol-20-one from the Hydrolysis Products of 'Sodium Pregnanediol Glucuronidate'. Nature 157, 438 (1946).

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