Crystallization and Identity of the Triose and Triosephosphate Dehydrogenases of Muscle

    Abstract

    THE triose (glyceraldehyde) dehydrogenase was studied by Green, Needham and Dewan1, who concluded from its distribution that it was a different enzyme from the triosephosphate dehydrogenase. We have succeeded in isolating and crystallizing the triose enzyme from rabbit skeletal muscle. After this was accomplished, we discovered that the crystals were in fact those of triosephosphate dehydrogenase. Their activity is actually some three hundred times greater with triosephosphate than with triose. It is therefore no longer necessary to assume the existence of triose dehydrogenase as a separate enzyme, as the two are identical.

    Access options

    Rent or Buy article

    Get time limited or full article access on ReadCube.

    from$8.99

    All prices are NET prices.

    References

    1. 1

      Green, D. E., Needham, D. M., and Dewan, J. G., Biochem. J., 31, 2327 (1937).

      CAS  Article  Google Scholar 

    2. 2

      Warburg, O., and Christian, W., Biochem. Z., 303, 40 (1939).

      CAS  Google Scholar 

    3. 3

      Needham, D. M., and Pillai, R. K., Biochem. J., 31, 1837 (1937).

      CAS  Article  Google Scholar 

    4. 4

      Needham, D. M., and Lu, G. D., Biochem. J., 32, 2040 (1938).

      CAS  Article  Google Scholar 

    5. 5

      Cori, G. T., Slein, M. W., and Cori, C. F., J. Biol. Chem., 159, 565 (1945).

      CAS  Google Scholar 

    6. 6

      Bailey, K., Biol. Bull., 77, 303 (1939); Nature, 145, 934 (1940).

      Google Scholar 

    7. 7

      Baranowski, T., Z. physiol. Chem., 260, 43 (1939).

      CAS  Article  Google Scholar 

    Download references

    Rights and permissions

    Reprints and Permissions

    About this article

    Cite this article

    Crystallization and Identity of the Triose and Triosephosphate Dehydrogenases of Muscle. Nature 156, 630–631 (1945). https://doi.org/10.1038/156630c0

    Download citation

    Further reading

    Comments

    By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.