Letter | Published:

Detection of Chemotherapeutics in Thin Sections of Tissue by the Aid of Fluorescence Microscopy

Nature volume 155, page 109 (27 January 1945) | Download Citation



IT has long been known that, thanks to their property of showing fluorescence in ultra-violet light, certain substances can be demonstrated even in very low concentrations. As a large number of the common drugs show fluorescence, it would appear reasonable to endeavour to demonstrate their presence also in tissues by the aid of fluorescence microscopy. The method has not been used very greatly, however, in view of the fact that the natural fluorescence of the tissues is so strongly blue that it masks the fluorescence of the drugs administered. In addition, the histological methods used have not excluded the possibility of changes in the locality and concentration of the substance sought during the actual preparation.

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  1. 1.

    , âœÃ¼ber die Eigenfluorescenz tierischer Gewebe mit besonderer Berücksichtigung der SÃugetierniereâ (Stockholm, 1944 ).

  2. 2.

    , âœNachweis von Prontosil solubile in histologischen Gewebsschnitten mit Hilfe der Fluorescenzmikroskopieâ, Acta phys. Scand. (1944).

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  1. Medico-Physiological Laboratory, Medical Clinic I, Karolinska sjukhuset, Stockholm.



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