Letter | Published:

Phase Difference Microscopy

Nature volume 154, page 672 (25 November 1944) | Download Citation

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Abstract

THE microscope does not reveal detail in uncoloured, transparent specimens even though it is known to be present and of sufficient size to be resolvable. When the detail includes regions of different refractive index, phase differences will be involved in light passing through the specimen, and if these phase differences are changed into intensity differences, the detail may be seen since the eye is sensitive to the latter although not to the former.

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References

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    , J. Roy. Micro. Soc., 150 (1905). , J. Roy. Micro. Soc., 388 (1904); 152 (1905).

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    , Z. tech. Phys., 16, 454 (1935).

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    , and , Naturwiss., 29, 49–61 (1941).

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Affiliations

  1. Research Division, Spencer Lens Company, Buffalo, N.Y.

    • OSCAR W. RICHARDS

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DOI

https://doi.org/10.1038/154672a0

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