A Modification of the Method for Estimating the Anti-Bacterial Activity of Fungi that are Difficult to Grow on Liquid Media

Abstract

RECENTLY¹ we published a method which was specially designed to estimate the anti-bacterial activity of certain fungi, for example, the Basidiomycetes, which are difficult to grow on liquid media. Briefly, the method consisted in cutting a disk from a growing fungus colony and dropping it into a plate of warm agar which had been bulk-seeded with bacteria. In case of a positive result a zone of bacterial inhibition was produced round the disk. The method stated that "all disks are cut at approximately the same distance from the edge of the colony". An attempt to determine what distance from the edge of the colony would give the best result brought out certain objections to the method and led to the present modification.