A Simple Fluorometer of the Duboscq Type


VARIOUS fluorometric methods are employed in determining riboflavin and thiamin. The fluorescent substance is freed, and in the final stage is compared with solutions of known concentration. Weisberg and Levin1 recommended the use of a block-comparator, but later authors2,3,4 employ a photo-cell connected with a galvanometer for testing the intensity of fluorescence. These photocell arrangements, however, are very expensive.

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  1. 1

    Weisberg, S. M., and Levin, J., Ind. and Eng. Chem., Anal Ed., 9 (1937).

  2. 2

    Schumacher, A. E., and Heuser, G. F., Ind. and Eng. Chem., Anal Ed., 12 (1940).

  3. 3

    Conner, R. T., and Straub, G. J., Ind. and Eng. Chem., Anal Ed., 13 (1941).

  4. 4

    Najjar, V. A., J. Biol. Chem., 141 (1941).

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KOCH, W. A Simple Fluorometer of the Duboscq Type. Nature 154, 239 (1944) doi:10.1038/154239a0

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