Isolated Nerve–Muscle Junction


A SINGLE muscle fibre with its nerve supply (Fig. 1) has been dissected from the M. adductor longus of the frog (Hyla aurea). This preparation survives for as long as twenty–four hours at a temperature of 18–20°C., and electric potential changes have been recorded from some twenty of these isolated nerve–muscle junctions during activity. In most experiments the muscle fibre was kept in saline at a paraffin interface while the nerve was lifted into the paraffin and stimulated there. A 50–µ diameter platinum wire served as leading electrode at the junction and was insulated except where it made contact with the muscle fibre. It was moved to different positions along the muscle fibre by means of a micrometer adjustment. The second leading electrode was in the saline below the fibre and acted thus as a diffuse lead.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    J. Neurophysiol., 4 (July, 1941).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

KUFFLER, S. Isolated Nerve–Muscle Junction. Nature 148, 315 (1941).

Download citation


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.