Abstract
A STRONG solution of pure cytochrome c obtained by the method which we have previously described1 shows in the oxidized state an absorption spectrum of the parahæmatin type. In a very much higher concentration and in a thick layer this compound reveals the two additional feeble absorption bands at about 695 mμ and 625 mμ discovered by Yakushiji2. The absorption spectrum of oxidized cytochrome c changes with the hydrogen ion concentration2,3. In this respect, cytochrome c resembles other trivalent hæematin compounds such as methæmoglobin, parahæmatin and hæematin. It is known, for example, that a parahæmatin compound has a characteristic absorption spectrum only within a certain limited range of hydrogen ion concentration4. On acidifying it or on making it alkaline the absorption spectrum of parahæmatin changes respectively to those of free acid and free alkaline hæmatins.
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References
Keilin, D., and Hartree, E. F., Pror. Roy. Soc., B, 122, 298 (1937).
Yakushiji, E., Acta Phytochimica, 10, 127 (1937).
Theorell, H., and Åkesson, Å., Science, 90, 67 (1939).
Keilin, D., Proc. Roy. Soc., B, 100, 129 (1926).
Sreenivasaya, M., and Pirie, N. W., Biochem. J., 32, 1707 (1939).
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KEILIN, D., HARTREE, E. Properties of Cytochrome c. Nature 145, 934 (1940). https://doi.org/10.1038/145934a0
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DOI: https://doi.org/10.1038/145934a0
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