Vernalization of Fragments of Embryo Tissue


EMBRYOS of cereal grains, separated from the endosperm and cultured on a medium containing sugar, can be vernalized by exposure to a temperature of 1° C. in the same way as whole grains1,2,3,4. In order to locate more precisely the tissues concerned in the process, embryos of Petkus winter rye were mutilated in various ways before vernalization treatment. In this preliminary experiment only two embryos were subjected to each type of mutilation.


  1. 1

    Gregory, F. G., and Purvis, O. N., NATURE, 138, 249 (1936).

  2. 2

    Gregory, F. G., and Purvis, O. N., Ann. Bot., N.S. (2), 237 (1938).

  3. 3

    Gregory, F. G., and de Ropp, R. S., NATURE, 142, 481 (1938).

  4. 4

    Konovalov, I. N., C.R. Acad. Sci., U.R.S.S., 16, 381 (1937).

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PURVIS, O. Vernalization of Fragments of Embryo Tissue. Nature 145, 462 (1940).

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