Physical Characterization of Calf Thymus Nucleohistone


WE have characterized calf thymus nucleohistone, prepared by modified Huiscamp1 methods, by observation of its sedimentation velocity, sedimentation equilibrium, diffusion, viscosity and electrophoresis. Between pH 5·2 and pH 9·0 in buffers containing 5 per cent sodium chloride it is monodisperse, and close to p H 10·4 is irreversibly transformed into a homogeneous material having a smaller sedimentation constant than the original nucleohistone. In these buffers, the reciprocals of the sedimentation constants of the nucleohistone and its transformation product are linear functions of the protein concentration, and the limiting values of the sedimentation constants for zero protein concentration have been found.

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  1. 1

    Huiscamp, W., Z. physiol. Chem., 32, 145 196 (1901).

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CARTER, R., HALI, J. Physical Characterization of Calf Thymus Nucleohistone. Nature 144, 329–330 (1939).

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