Existence of Several Active Components in Crude Pepsin Preparations


THE original solubility measurements on crystalline swine pepsin led to the conclusion that the crystals consisted of a single pure protein or several closely related proteins1. Since that time, it has become apparent in this and other laboratories2,5 that pepsins prepared from the various commercial products or from pepsinogen differ in solubility, specific proteolytic activity and stability. Solubility measurements have now been made on various partially purified pepsin preparations in order to determine the cause of these variations.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Northrop, J. Gen. Physiol., 13, 739 ( 1930).

    CAS  Article  Google Scholar 

  2. 2

    Northrop, J. Gen. Physiol., 17, 165 ( 1933). Herriott, J. Gen. Physiol., 21, 501 (1938). Agren and Hammarsten, Enzymologia, 4, 49 ( 1937). Philpot and Small (unpublished results, see Tiselius et al.5). Steinhardt, Cold Spring Harbor Symposia on Quantitative Biology, 6, 301 ( 1938). Holter, Z. physiol. Chem., 169, 1 ( 1931). Northrop, J. Gen Physiol., 15, 29 ( 1932).

    CAS  Article  Google Scholar 

  3. 3

    Kunitz and Northrop, C.R. Trac. Lab. Carlsberg, 22, 288 ( 1938); Cold Spring Harbor Symposia on Quantitative Biology, 6, 325 (1938).

    CAS  Google Scholar 

  4. 4

    The hæmoglobin method used in this work has been described by Anson, J. Gen. Physiol., 22, 79 (1938), and is a modification of the previously described method (Anson and Slirsky, J. Gen. Physiol., 16, 59; 1932). The modified method gives results which are 1015 per cent higher than the old method and not lower as reported by Anson. The specific activity of 0·35 now found for the A component therefore represents an activity of about 0.31 by the method previously used.

    CAS  Article  Google Scholar 

  5. 5

    Tiselius, Henschen and Svensson, Biochem. J., 37, 1814 (1938).

    Article  Google Scholar 

Download references

Author information



Rights and permissions

Reprints and Permissions

About this article

Cite this article

DESREUX, V., HERRIOTT, R. Existence of Several Active Components in Crude Pepsin Preparations. Nature 144, 287–288 (1939). https://doi.org/10.1038/144287b0

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.