Letter | Published:

A Chemical Method of Assay of Insulin

Nature volume 143, page 686 (22 April 1939) | Download Citation



IN a recent communication1, Vogelenzang suggests the possibility of an iodometric method for estimating the physiological potency of insulin preparations. A similar suggestion was previously made by Brand and Sandberg2. Jensen, Schock and Sollers3, however, pointed out that the success of an iodometric titration of insulin is dependent upon the amount of impurities which will react with iodine. Since iodine reacts upon certain chemical groupings which are by no means confined solely to the insulin molecule, it is doubtful whether the physiological potency of insulin preparations can be reliably determined by iodometrie titration. Blatherwick and associates4 have arrived at the same conclusion.

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  1. 1.

    , NATURE, 143, 161 (1939).

  2. 2.

    , and , Proc. Soc. Exp. Biol. and Med., 23. 313 (1926).

  3. 3.

    , , and , J. Biol. Chem., 98, 93 (1932).

  4. 4.

    , , , , and , J. Biol. Chem., 72, 57 (1927).

  5. 5.

    , "Insulin, Its Chemistry and Physiology". The Commonwealth Fund, New York, 1938.

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  1. Biochemical Laboratory, Squibb Institute for Medical Research, New Brunswick, N.J. Feb. 21.

    • H. JENSEN


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