Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Present Condition of a Twelve-Year Old Pure Strain of Carcinoma Cells in vitro

Abstract

ON February 23, 1927, five small fragments of a mouse adenocarcinoma (Frankfurt strain of Ehrlich) were cultivated as hanging drop cultures1. With slight modifications, the culture medium used has remained practically the same throughout this period of twelve years. The medium used for cover-glass cultures consisted of one volume of a plasma mixture (equal parts of chick and rat plasma) and one volume of embryonic tissue juice of chick. For cultures in Carrel flasks, the solid medium was composed of equal parts of dilute chick and rat plasma coagulated by a drop of embryonic tissue juice; the supernatant liquid phase, 0.5 c.c., consists of 20 per cent embryonic tissue juice (chick), 20 per cent rat serum and 60 per cent ‘Tyrodc’ solution.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Z. Krebsforsch., 25, 89 (1927).

  2. Fischer, A., "Gewebezüchtung" (Munich, 1930).

    Google Scholar 

  3. Ann. Anat. path., 18, 665 (1931).

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

FISCHER, A., DAVIDSOHN, F. Present Condition of a Twelve-Year Old Pure Strain of Carcinoma Cells in vitro. Nature 143, 436–437 (1939). https://doi.org/10.1038/143436b0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/143436b0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing