Abstract
EVIDENCE for the earlier suggested1 participation of a flavin compound in pyruvic acid oxidation by Bacterium Delbrückii has been obtained by a procedure similar to that used by Warburg and Christian2 to remove the prosthetic group (flavin-adenin dinucleotide) from amino-acid oxidase. Phosphate extracts derived from the lactic acid bacteria were repeatedly precipitated with 50 per cent ammonium sulphate at pH. 3 at 1–2° C. By this treatment a protein fraction was obtained, which on addition of thiamin (vitamin B1) pyrophosphate only1, did not catalyse pyruvic acid oxidation, but did so on addition of thiamin pyrophosphate together with flavin-adenin dinucleotide, kindly supplied to me by Prof. O. Warburg.
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References
Lipmann, Enzymologia, 4, 65 (1937).
Warburg and Christian, Biochem. Z., 298, 150 (1938).
Lipmann and Perlmann, J. Amer. Chem. Soc., 60, 2574 (1938).
Straub, NATURE, 143, 77 (1939).
Straub, Corrau and Green, NATURE, 143, 119 (1939).
Adler, Euler and Hellström, Sv. Vat. Akad. Ark. Kami, 12, 1 (1937).
Dewan and Green, Biochem. J., 32, 626 (1938).
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LIPMANN, F. Flavin Component of the Pyruvic Acid Oxidation System. Nature 143, 436 (1939). https://doi.org/10.1038/143436a0
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DOI: https://doi.org/10.1038/143436a0
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