DURING spectroscopic study of a flavoprotein compound in heart muscle preparation, it was noticed that two diffuse absorption bands of the pigment remained always perceptible, however dilute was the preparation or thin the layer examined. Further observations have revealed that these two bands remain perceptible even in the absence of muscle preparations. Moreover, examination with a small dispersion spectroscope of any source of artificial light shows the presence of two diffuse absorption bands lying at about 495 mÂµ. and 455 mÂµ. The first band is more distinctly visible at a lower intensity of light, while the second band can be more easily perceived when the light passes through a suitable filter such as a solution of ammoniacal copper sulphate or Wratten filter D.
Gibson, K. S., and Tyndall, E. P. T. . Bur. Stand. Sci. Papers, 10, 131 (1923).
Fedorow, N. T., and Fedorowa, W. I., C.R. Acad. Sci., U.R.S.S. 2, 377 (1936).
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Brunner, O., and Baroni, E., Sitzber. Akad. Wiss. Wien, Math. natw. Klasse, IIC., 145, 484 (1936).
Brunner, O., Baroni, E., and Kleinau, W., Z. physiol. Chem., 236, 257 (1935).
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KEILIN, D., SMITH, E. Direct Perception of Pigment in the Nerve Tissue of Human Retina. Nature 143, 333 (1939). https://doi.org/10.1038/143333a0
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