WE have begun to study systematically extracts of endocrine glands containing hormones of a protein nature, especially extracts of the anterior pituitary lobe (ALH) of cattle. For technical reasons very dilute extracts were used: 1 gm. of the dry powder, previously kept in vacuo, was shaken for 4 hours with 20 ml. of a 0.0001 N NH4Cl solution and then filtered. The pH. of the filtrate was kept at about 6.2.
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HANDOVSKY, H., HAUSS, L. Determination of rH in Endocrine Extracts of a Protein Nature. Nature 143, 283 (1939). https://doi.org/10.1038/143283a0
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