Letter | Published:

Ninhydrine Reaction in the Quantitative Determination of Different Amino Acids

Nature volume 142, page 754 (22 October 1938) | Download Citation

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Abstract

IN the so-called ninhydrine reaction there is formed, in addition to carbon dioxide, an aldehyde corresponding to the particular amino-acid present. When the reaction is carried out so that the formation of aldehyde is as quantitative as possible, the amino-acid can be determined by the aldehyde. We have made this method quantitative for α-alanine, which gives acetaldehyde1. The ninhydrine reaction can also be applied to the quantitative determination of certain other amino-acids. Thus one of us (Laine) has developed the method for the determination of leucine by means of isovaleraldehyde formed in the reaction2. We are continuing our work for the determination of other amino acids in the same manner.

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References

  1. 1.

    Skand. Archiv Physiol., in the Press.

  2. 2.

    Suomen Kemistilehti, B, in the Press.

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Author information

Affiliations

  1. Biochemical Institute, Helsinki. Sept. 8.

    • ARTTURI I. VIRTANEN
    •  & T. LAINE

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DOI

https://doi.org/10.1038/142754c0

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