Letter | Published:

Colchicine and Acenaphthene as Polyploidizing Agents

Nature volume 142, page 753 (22 October 1938) | Download Citation



FROM the communication by B. R. Nebel1 in NATURE of August 6 reporting that acenaphthene solution does not induce chromosome doubling in Tradescantia, I believe that he is not well acquainted with the method by which I worked. In my first paper2 I described the method as follows: “Soaking seeds from Triticum vulgare, Tr. monococcum, and Secale cereale in saturated aqueous solution of acenaphthene, with excess of crystals (sublimated particles), during two days and then putting them into Petri dishes, watered with the same solution, we found that the seedlings react morphologically in the same way as they react to colchicine solution” (p. 198). In the treated material polyploid cells and sectors were found. Excess of crystals was necessary (which act in the form of sublimated particles), since saturated solution alone was not sufficient to induce chromosome doubling. (I shall not quote here the further elaborations of the method3 described in my paper quoted by Nebel, since it was published in NATURE.)

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  1. 1.

    , NATURE, 142, 257 (1938).

  2. 2.

    , C.R. Acad. Sci. U.S.S.R., 19, 197–199 (1938).

  3. 3.

    , NATURE, 141, 1144–1145 (1938).

  4. 4.

    , Current Science, 6, 549–552 (1938).

  5. 5.

    , Current Science, in the Press.

  6. 6.

    , Current Science, in the Press.

  7. 7.

    , C.R. Acad. Sci. U.S.S.R., in the Press.

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  1. Institute of Genetics, Academy of Sciences of U.S.S.R., Moscow.



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