Biological Fixation of Nitrogen


THE oxime which is formed in the root nodules during the nitrogen fixation of legume bacteria1 has at last been isolated and characterized. The oxime precipitates according to Foreman's method, which fact already indicates it to be the oxime of a dicarbonic acid. Extracting the oxime with ether, we succeeded in preparing the copper salt from it. The analysis of this salt showed that the compound is the oxime of oxalacetic acid. Thus the formation of aspartic acid in the biological fixation of nitrogen via the oxime of oxalacetic acid has been finally confirmed.


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VIRTANEN, A., LAINE, T. Biological Fixation of Nitrogen. Nature 142, 165 (1938).

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