Abstract
IN the attempt to isolate the enzyme uricase from pig liver, I have obtained a preparation 550 times purer than the enzyme powder used as starting material. The purified preparation contains 0·15–0·18 per cent Fe, 14·4 per cent N, traces of copper, and no cobalt or manganese. It is almost colourless, and therefore is not a hæmin derivative. It is insoluble in water, almost insoluble in phosphate buffer pH. 7·4, and soluble in borate buffer pH 10. Truszkowski1 has already shown that uricase is soluble in alkaline solution.
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References
Truszkowski, R., Biochem. J., 28, 62 (1934).
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DAVIDSON, J. Purified Uricase. Nature 141, 790 (1938). https://doi.org/10.1038/141790a0
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DOI: https://doi.org/10.1038/141790a0
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