Letter | Published:

Quantitative Measurement of Vitamin B1 the Thiochrome Reaction

Nature volume 141, page 1141 (25 June 1938) | Download Citation

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Abstract

IN 1936, Jansen1 first suggested that vitamin B1 could be measured quantitatively by conversion into thiochrome with alkaline ferricyanide and measurement of the fluorescence of the thiochrome. Since that time, information has been collected on the use of this method for the assay of vitamin B1 in urine 2,3,4 and in other materials including foodstuffs5. Evidence, however, is now accumulating that, although results which compare closely with the biological assay can be obtained with crystalline vitamin B1 and acid-clay adsorbates of the vitamin when the precautions described elsewhere5 are exercised, the assessment by the chemical method on certain foodstuffs is significantly lower than the biological results.

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References

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    , Rec. Trav. chim., 55, 1046 (1936).

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Affiliations

  1. Research Laboratories, Vitamins, Ltd., 23 Upper Mall, London, W.6. May 24.

    • MAGNUS PYKE

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DOI

https://doi.org/10.1038/1411141a0

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