Abstract
Stimulation of dopamine (DA) receptors in the striatum evokes a number of alterations in motor behavior in rats, as well as causing several alterations in cellular physiology, including changes in membrane potential, cell excitability, afferent drive, and electrotonic coupling. One cellular property that is potently modulated by DA stimulation is electrotonic coupling, a process shown to subserve motor pattern generation. To examine whether electrotonic coupling plays a role in mediating a specific set of DA receptor-mediated motor behaviors, we tested the effects of two inhibitors of gap junction conductance, carbenoxolone (CARB) and anandamide (AEA), on apomorphine (APO)-induced motor responses. We then used intra-striatal infusions of CARB to determine the role of electrotonic coupling specifically in the ventral striatum in the expression of APO-induced behaviors. APO (2.5–3.0 mg/kg, i.p.) significantly increased motor activity (a composite score) and the frequencies of oral and sniffing stereotypies. APO also disrupted grooming initiation and completion. APO-induced oral stereotypies were selectively blocked by systemic administration of CARB (7.0, 35.0 mg/kg). Moreover, although CARB alone disrupted the initiation and completion of grooming sequences, it also partially normalized APO-induced disruptions in grooming. AEA (0.5, 1.5 mg/kg) also blocked APO-induced oral stereotypies at the higher dose, but differed from CARB in that it did not restore normal grooming behaviors but, instead, appeared to “release” locomotion. Bilateral infusion of carbenoxolone (50 pmol) into the ventral striatum also blocked the oral stereotypies induced by systemic APO. We conclude from these and previous experiments that gap junctions play an important role in normal motor behavior, and furthermore that disruption of motor behavior in the form of oral and sniffing stereotypies associated with systemic APO administration may be a consequence of this heightened electrotonic coupling in the striatum. These results may be relevant to diseases and pharmacotherapies associated with disruptions of motor and possibly cognitive sequencing.
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Main
Adaptive behaviors, both learned and spontaneous, consist of sequences of “micro” movements executed by closely-related muscle groups. There is evidence that the initiation and execution of individual movements, such as oral movements involved in feeding, as well as the linking of these movements into a behavioral sequence, depend upon neuronal networks within the striatum (Aldridge and Berridge 1998; Aldridge et al. 1993; Cromwell and Berridge 1996; Matsumoto et al. 1999). Motor initiation and sequencing are also potently modulated by dopamine (DA) transmission in the striatum, as evidenced by the disruptive effects of DA denervation on both initiation and sequencing of movements. Moreover, downregulation or antagonism of DA D1 receptors with genetic, antisense, or pharmacologic techniques can disrupt sequencing per se (i.e. individual behaviors are expressed, but not sequentially (Berridge and Aldridge 2000a,b; Cromwell et al. 1998). Consistent with this, D1 receptor stimulation promotes the completion of complex behavioral sequences such as grooming (Molloy and Waddington 1984a). On the other hand, whereas blockade or genetic inactivation of D2 receptors impairs the initiation of movements (Fowler and Liou 1994; Horikawa et al. 1997; Sibley 1999), stimulation of D2 receptors by non-selective or D2-selective agonists produces repetition of simple behaviors such as licking, gnawing, or sniffing stereotypy (Abrams and Bruno 1992; Beninger et al. 1991; Braun and Chase 1986; Byrnes et al. 1994; Delfs and Kelley 1990). Thus, in the context of behavioral sequencing, the simple ‘repetitive’ stereotypy (Berridge and Aldridge 2000a) produced by D2 receptor over-stimulation in the striatum (Delfs and Kelley 1990) can be seen as the inability to release one ‘simple’ behavior in order to allow initiation of the next simple behavior in a normal, adaptive sequence (Pedro et al. 1994; Szechtman et al. 1999).
One means by which neuronal networks may interact within the striatum is via communication among clusters of functionally related striatal neurons. Such a network interaction may be mediated via gap junctions. It is well-established that electrotonic transmission between developing neurons is important in the formation and stabilization of functional networks (Bannerman et al. 2000; Peinado et al. 1993; Roerig and Feller 2000). However, there is also evidence that in the adult brain, electrotonic coupling directly mediates forms of neuronal network activity (Bou-Flores and Berger 2001; Jefferys 1995; Perez Velazquez and Carlen 2000) which, in turn, contribute to rhythmic behavioral patterning (Bou-Flores and Berger 2001; Rothwell 1998). Thus, it is likely that motor patterning may involve activity within neuronal networks coordinated via gap junctions. In the striatum, cells express connexins, the protein building blocks of gap junctions (Belluardo et al. 2000; Bennett et al. 1999). Striatal neurons also exhibit dye coupling, the transfer of dye molecules between neurons through electrotonic junctions (Cepeda et al. 1989). Moreover, like behavioral sequencing, dye coupling between neurons in the striatum is potently modulated by DA receptor activation (Cepeda et al. 1989; Onn and Grace 1994, 1995, 1999). In the present study, it was postulated that electrotonic coupling within the striatum might subserve network processes that, in turn, modulate behavioral sequencing. Therefore, disruption of gap junctions may ultimately interfere with such behavioral sequencing We examined whether gap junctions may play a role in behavioral patterns by testing the effects of gap junction inactivators on the motor behavior elicited by the DA agonist apomorphine. Apomorphine administration is known to evoke a broad range of DA-mediated behaviors, including hyperlocomotion, rearing, sniffing, and stereotypy. In addition, we found acute apomorphine administration to lead to a marked increase in both the incidence and extent of dye coupling among neurons in the dorsal striatum (Onn and Grace 1994). The response to apomorphine was examined during administration of the gap junction inactivators carbenoxolone (CARB) and anandamide (AEA) because of their shared effectiveness in blocking gap junction conductance or electrotonic coupling in brain tissue but lack of similarity in their other cellular or behavioral effects. Carbenoxolone is a moderately lipophilic glycyrrhetinic acid derivative that has been shown to act directly on gap junctions in brain and other tissues to reduce conductance by up to 80%, although the exact mechanism underlying this effect remains to be determined (Davidson and Baumgarten 1988; Rozental et al. 2001). It inhibits gastric pepsins (Roberts and Taylor 1980) and has a number of mineralocorticoid actions (Armanini et al. 1982), including inhibition of 11-β-dehyrogenase and 11-oxoreductase (Stewart et al. 1990). Anandamide, on the other hand, is a highly lipophilic arachidonic acid-derived ethanolamide that has been shown to block gap junction transmission (Boger et al. 1999), more specifically, in striatum (Venance et al. 1995). At doses at or above 10 mg/kg it acts as an agonist at central cannabinoid CB1 receptors, producing catalepsy and hypothermia (Costa et al. 1999; Crawley et al. 1993; Fride and Mechoulam 1993). It also acts at peripheral CB1 (De Petrocellis et al. 1998) and vanillin receptors (De Petrocellis et al. 2001), actions possibly related to its analgesic effects. Thus, although actions of CARB and AEA have not been fully characterized, there appears to be little overlap in their pharmacological or physiological effects besides gap junction inactivation. Therefore, we used these drugs to determine if a class of dopamine receptor-mediated behaviors depends on electronic coupling in general and, more specifically, within the striatum.
MATERIALS AND METHODS
Subjects
Naïve male Sprague-Dawley rats three to six months of age were double-housed in a 12:12 h light/dark cycle, lights on at 7 A.M., with food and water available ad libitum (Hilltop Laboratories, Scottdale, PA). All animals were tested during the first 4 h of the dark cycle (between 7 and 11 P.M.) All procedures were conducted according to the NIH Guide for the Care and Use of Laboratory Animals and were approved by the University of Pittsburgh Institutional Animal Care and Use Committee.
Drugs
Carbenoxolone (Sigma, St. Louis, MO) and anandamide (RBI, Natick, MA) were dissolved by adding 10 microliters of 70% ethanol then bringing the solution to a volume of 1.0 mL with 0.05 M saline. This mixture was then placed in an ultrasonic bath for 10–15 min until the drug was dissolved or completely suspended. Apomorphine hydrochloride (RBI) was dissolved in distilled water. Systemic injections were given at 1 ml/kg. Controls were injected with the vehicle used for each drug.
Measurement of Motor Activity and Stereotypy
Rats were tested during the dark cycle in an 46 l × 20 w × 20 h (cm) polycarbonate tub with the floor divided into quadrants. The motor activation scale, adapted from Byrnes et al. (1994), was modified based on the dose and time-dependent effect of apomorphine (APO) on motor activity as determined in pilot experiments and reported by others (Abrams and Bruno 1992; Braun and Chase 1986). The progression of APO-induced motor activity followed the following scale:
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1
inactive
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2
intermittent head or forepaw movement; normal exploratory behavior within a quadrant
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3
bouts of continuous head (including sniffing) or forepaw movement; locomotion involving one or two quadrants
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4
locomotion and sniffing across two2 or more quadrants
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5
continuous locomotion and sniffing
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6
continuous sniffing localized to one area of the tub; intermittent oral stereotypy
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7
continuous oral stereotypy (licking, mouthing, gnawing)
Simple repetitive stereotypies, including sniffing, licking, mouthing, and gnawing, were also scored individually as not present (0), intermittent (1; bouts < 15 s), or constant (2; bouts > 1 min). Grooming behaviors, including paw wash, face/head wash, and hindflank wash were each recorded. Grooming sequence initiation and grooming sequence completion were scored post-hoc using criteria based on those of Aldridge and Berridge (Aldridge and Berridge 1998; Berridge and Aldridge 2000a, b). Specifically, a paw wash initiated as the first grooming behavior in a scoring period was considered “grooming sequence initiation” (Phase I). If paw wash was followed by face or head wash (Phases II or III) and flank wash, then the flank wash was scored as “grooming sequence completion” (Phase IV).
Experiment 1: The Effect of Systemic Administration of Gap Junction Inactivators on Apomorphine-induced Stereotypy
Carbenoxolone and AEA were tested independently. For each gap junction inactivator, rats were divided into five groups. The VEH-VEH group received the vehicle of the gap junction inactivator (VEH1) followed by injection of the vehicle for apomorphine (VEH2). The second group received CARB (35.0 mg/kg) or AEA (1.5 mg/kg) followed by VEH2. The third group received VEH1 followed by APO. The fourth group received a lower dose of CARB (7.0 mg/kg) or AEA (0.5 mg/kg) then APO. The fifth group received the higher dose of CARB (35.0 mg/kg) or AEA (1.5 mg/kg) prior to receiving APO. APO from different batches appeared to have different efficacies, so the dose was adjusted from 2.5–3.0 mg/kg to produce equivalent levels of stereotypy among VEH1-APO groups. All drugs were injected intraperitoneally (i.p.). The two injections were spaced by 15 min.
Experiment 2: The Effect of Intra-striatal Infusion of Carbenoxolone on Stereotypy Induced by Systemic Administration of Apomorphine
Guide cannulae (28 gauge., Plastics One) were implanted bilaterally into the central ventral striatum at 0.5–1.0 mm anterior to Bregma, 1.5–2.5 mm lateral to the midline and 5 mm ventral to the skull surface according to the atlas of Paxinos and Watson (1986). Animals were allowed to recover for three days prior to testing. During this time and before surgery they were habituated to the restraint procedure used for intracranial injection. During testing, animals were gently restrained by slipping two forefingers over the snout. Needles (30 gauge) filled with carbenoxolone (10 μM) were then inserted into the cannulae. Needles extended 1.0 mm beyond the tip of the guide cannulae. A volume of 0.5 microliters per side of CARB or VEH1 was infused over 2 min. The needles were then left in place and the inlet tubing was cut close to the inlet of the cannulae. Ten minutes following the intra-striatal infusion, APO (3.0 mg/kg) was administered systemically (i.p.).
Statistical Analyses
Effects of each gap junction inactivator on spontaneous (baseline) and APO-induced activity (activity score), ambulation (number of quadrant crossings), and oral and sniffing stereotypies were analyzed independently for each dependent variable by first applying a mixed ANOVA using Drug condition as a factor and Time after APO administration as the repeated measure. In cases in which there was no significant effect of Time or Time X Drug interaction, the dependent variable was averaged over the period from 10–25 min after APO administration and a 1-way ANOVA with Tukey's post-hoc comparisons was used to test for differences among drug conditions. The proportion (frequency) of animals exhibiting grooming initiation and completion were compared across conditions with the binomial test. All statistical tests were conducted using SPSS software (SPSS, Inc., Chicago, IL).
RESULTS
Experiment 1a. Effects of Systemic Administration of CARB on the Behavioral Effects of APO
The following groups were included in the analyses described below: VEH1-VEH2 (n = 7), CARB (35.0 mg/kg)-VEH2 (n = 11), APO-VEH2 (n = 12), CARB (7.0 mg/kg)-APO and CARB (35.0 mg/kg)-APO (n = 10).
Locomotion and Simple Stereotypies
A 2-way ANOVA analyzing the effects of drugs over the period from 5 to 25 min following APO administration revealed neither a main effect of Time nor a Time X Drug interaction (Figure 1 , panel a). Therefore, the effects of drug treatment were analyzed with a 1-way ANOVA using the average motor activity score for the period of 10–25 min following APO administration. This analysis revealed a significant effect of drug treatment (F4,43 = 23.4, p < .001; Figure 1, panel b). Tukey's post-hoc comparisons revealed that CARB-VEH animals did not significantly differ from controls but APO animals showed a significant increase in motor activity. CARB dose-dependently blocked APO-induced motor activity with the lower dose being not significantly different from either control or APO, and the higher dose being significantly lower than APO and not different than control. Analysis of the concurrent effects on quadrant crossings (a measure of ambulatory activity) and sniffing and oral stereotypies further revealed that while APO had no net effect on quadrant crossings (Figure 2 , panel a), it significantly increased biting (Figure 2, panel b), licking (Figure 2, panel c), and sniffing (Figure 2, panel d) stereotypies. This may have resulted from a tendency to increase locomotion concurrent with the emergence of stereotypies that competed with locomotion. Thus, the increase in overall motor activation appeared to be due to APO-induced oral and sniffing stereotypies. CARB had no systematic effect on locomotion following VEH2 or APO (Figure 2, panel a). However, it dose-dependently blocked APO-induced biting (Figure 2, panel b) and licking (Figure 2, panel c) stereotypy (Tukey's comparisons, CARB 7-APO not different from VEH-VEH or VEH-APO, CARB 35-APO vs. VEH-APO, p < .05; vs. VEH-VEH, p > .4) and also significantly reduced sniffing stereotypy (CARB-APO vs. VEH-APO, p < .05; Figure 2, panel d). Thus, it appeared that the reduction in licking, biting, and sniffing resulted in a significant reduction in overall motor activation.
Grooming
In the present experiment, control (VEH-VEH) animals initiated and completed grooming sequences during testing (Figure 3 ), but spent relatively little time grooming overall (data not shown). Thus, grooming was scored as having been initiated (with a paw and head wash) and, in those who initiated, completed with a body (flank) wash. The proportion of all animals tested that displayed grooming initiation alone (paw washing only; Figure 3, solid black area) or initiation and completion of a grooming sequence (Figure 3, hatched area) was recorded for each drug condition. Grooming initiation and completion were affected similarly by drug condition. Grooming initiation and completion were significantly reduced by CARB (CARB-VEH), and more markedly by APO (VEH-APO; Figure 3; p < .05, binomial comparisons of CARB-VEH and VEH-APO with VEH-VEH). Interestingly, pretreatment with CARB (35.0 mg/kg) blocked the APO-induced disruption of grooming initiation and completion (i.e. grooming sequencing in the CARB-APO condition was higher than in the VEH-APO condition, and not different from the VEH-VEH condition, binomial test).
Experiment 1b. Effects of Systemic Administration of AEA on the Behavioral Effects of APO
The following groups were included in the analyses described below: VEH1-VEH2 (n = 9), AEA (1.5 mg/kg) VEH2 (n = 5), APO-VEH2 (n = 6), AEA (0.5 mg/kg)-APO (n = 5) and AEA (1.5 mg/kg)-APO (n = 5).
Locomotion and Simple Stereotypies
As with Experiment 1a, no effects of time or interaction of time with drug treatment were revealed (Figure 4 , panel a). Motor activity during the 10–25 min period after injection of APO or its vehicle was significantly affected by drug treatment (F4,24 = 19.8, p < .001; Figure 4, panel b). APO produced a significant increase in activity, relative to VEH-VEH and AEA-VEH (Tukey's comparisons, p < .05) rats. AEA significantly reduced APO-induced motor activity but only at the higher dose; thus AEA 0.5 mg/kg-APO rats differed significantly from APO but not from controls, while rats pretreated with AEA 0.5 displayed activity levels not different from VEH-APO rats (Figure 4, panel b). Analysis of the concurrent effects on quadrant crossings and sniffing and oral stereotypies further revealed that, as in the CARB testing group, APO had no net effect on quadrant crossings (Figure 5 , panel a) but it significantly increased biting (Figure 5, panel b), licking (Figure 5, panel c) and sniffing (Figure 5, panel d) stereotypies. While AEA appeared to be effective in blocking APO-induced sniffing, AEA was similar to CARB in reducing APO-induced licking and biting stereotypy. In addition, at the 1.5 mg/kg dose, AEA also appeared to “release” the stimulation of locomotion by APO (Figure 5, panel a; Overall F = 1.75, p = .17, LSD two-tailed test for AEA 1.5 vs. VEH-VEH, p < .05).
Grooming
Similar to Experiment 1a, APO administration eliminated grooming initiation. However, unlike CARB, AEA was not effective at blocking this effect (data not shown).
Experiment 2. Effect of Intrastriatal CARB on the Behavioral Effects of Systemically- administered APO
In a total of 25 rats, cannula tip placements were within the ventral striatum ranging from 0.5 to 1.2 mm anterior to Bregma, 1.5 to 2.5 mm lateral to the midline and 4.5 to 7.0 mm ventral to the dural surface (Figure 6 ). Overall motor activity differed significantly across treatment groups (F3,21 = 23.0, p < .001; n = 5–8; Figure 7 ), with VEH-APO group differing significantly from all other groups, and CARB and CARB-APO not differing from controls. Thus, intrastriatal CARB sufficiently blocked the effects of systemic APO when assessed with a subjective motor activity score. This effect was not due to a net effect on quadrant crossings (Figure 7, panel b) but due mainly to the effects of intra-striatal CARB on oral stereotypies, with biting (Figure 7, panel c) and licking (Figure 7, panel c) being significantly reduced by CARB relative to APO alone (F3,22 > 88, p < .001; Tukey's comparisons). APO-induced sniffing stereotypy was not significantly affected by intra-striatal CARB pretreatment (Figure 7, panel d).
Grooming
Similar to Experiment 1a, APO administration disrupted grooming initiation (data not shown); however, there was no trend for a normalization by intrastriatal CARB.
DISCUSSION
In the present study, APO-induced simple oral and sniffing stereotypies were selectively blocked by systemic administration of two different gap junction inactivators. Moreover, local gap junction inactivation within the ventral striatum selectively blocked APO-induced oral stereotypies. We propose that the present data taken with previous studies on the pharmacology of APO-induced effects on behavior and electrotonic coupling in the striatum indicates that a major mechanism by which these gap junction inactivators blocked APO-induced stereotypy is by blocking a D2 receptor-mediated increase in electrotonic coupling.
The Specificity of the Effects of CARB and AEA on Electrotonic Coupling
As reviewed in the introduction, although both CARB and AEA have a number of physiological actions, the major action that these drugs have in common is the inactivation of gap junctions. The effects of systemic CARB administration that could interfere with the measurement of behavior include changes in kidney function and hypertension (Gomez-Sanchez and Gomez-Sanchez 1992). However these effects are achieved with chronic administration or with doses higher than those used in this study. Similarly, at doses several times higher than those used in this study, AEA produces ataxia, hypomotility (Aceto et al. 1998; Crawley et al. 1993), and acute hypotension (Garcia et al. 2001). Thus, given the relatively low doses used in the present study it is unlikely that the reductions in APO-mediated behaviors were secondary to these effects of CARB or AEA per se.
Because not only AEA, but also its inactivating enzyme fatty acid amide hydrolase, and multiple effector sites, including voltage gated calcium channels and CB1 receptors, are all located on basal ganglia neurons, it is important to consider mechanisms other than gap junction conductance that could have contributed to the ability of AEA to block APO-induced stereotypies. In addition to the inactivation of gap junction conductance, possible mechanisms of action of AEA include inhibition of voltage-gated calcium channels (Chemin et al. 2001), enhancement of specific potassium channels (White et al. 2001) and activation of CB1 receptors (Ameri 1999). In the present study, the effects of AEA on calcium and potassium channels would appear to be limited, since the major physiologic change produced by these actions, vasorelaxation and a decrease in systemic vascular resistance (Garcia et al. 2001), require doses significantly higher than those used to block APO-induced stereotypy. Similarly, large changes in calcium and potassium conductance in basal ganglia neurons or local cerebral blood vessels might be expected to cause marked effects on cerebral blood flow or basal ganglia function: effects that are not observed at the highest dose used in this study (Stein et al. 1998; present data). Thus, although there is evidence for interactions among AEA, calcium, and potassium channels, and junctions in mediating intercellular communication (Schweighofer et al. 1999), large changes in voltage-gated ion channels are unlikely to have mediated the behavioral effects observed in the present study.
There is considerable but not complete overlap in the brain regions enriched in AEA and its inactivating enzyme, fatty acid amide hydrolase (Di Marzo et al. 2000) and CB1 binding sites (Sanudo-Pena et al. 1999). However, the doses of AEA used in the present study are below those commonly used to produce CB1 receptor-mediated effects. Furthermore the behaviors produced by CB1 receptor stimulation (e.g. hypomotility) are quite different from those observed at the doses used in the present study (e.g. increases in locomotion). This is consistent with data showing that the effects of AEA on locomotor activity are biphasic, with increases in spontaneous locomotion or antagonism of cannabinoid-induced hypermotility occurring at doses < 0.01 mg/kg and hypomotility occurring at doses between 10–100 mg/kg. Importantly, a significant proportion of the motor effects of AEA, as with its effects on gap junction coupling, occurs independent of CB receptors (Ameri 1999; Di Marzo et al. 2000). Taking the above evidence together with the convergence of the effects of CARB and AEA on gap junction coupling and behavior, we conclude that electrotonic coupling may be a major substrate for interaction between AEA and DA systems in the basal ganglia. However, it is likely that this mechanism interacts with other actions of AEA (e.g. on CB1 receptors) in the basal ganglia to affect psychomotor behavior.
The Pharmacology of the APO-induced Stereotypies Blocked by AEA and CARB
The DA pharmacology of APO-induced stereotypies has been well-characterized. Specific stereotypies are mediated by specific regions within the striatum by an interaction between D1-type and D2-type DA receptors (Cameron and Crocker 1989; Kelly et al. 1975; Romero et al. 1995; Schmidt 1986). Whereas locomotion and head and sniffing stereotypies are mediated by a number of sub-circuits in dorsal and ventral striatum (Delfs and Kelley 1990; Koene et al. 1993; Cameron and Crocker 1989; Schmidt 1986), oral stereotypies such as biting and licking are regulated primarily by the medial or lateral ventral striatum (Delfs and Kelley 1990; Downes and Waddington 1993; Koene et al. 1993). Sniffing, biting and licking are characteristic effects of D2 receptor over-stimulation (i.e. produced by D2 agonists) (Davidkova et al. 1998; Fowler and Liou 1994; Wang et al. 2000), but also require a minimal level of D1 receptor stimulation (Beninger et al. 1991; Mashurano and Waddington 1986). Unlike most simple stereotypies and locomotion, grooming appears mainly to be a behavioral sequence mediated by D1 receptor stimulation in the central and dorsal striatum (Cromwell et al. 1998; Downes and Waddington 1993; Molloy and Waddington 1984b). The effects on grooming observed in the present study were intriguing, given the plausible role of gap junction in behavioral sequencing. The effectiveness of systemic AEA and CARB in blocking oral and sniffing stereotypies and the selective blockade of oral stereotypies by ventral striatal administration of CARB is consistent with the idea that D2 receptor-mediated increases in gap junction conductance (Onn and Grace 1994) are necessary in the expression of these stereotypies.
The Role of Gap Junctions and Dopamine in Signal Flow across Cortical-basal Ganglia Circuits and the Regulation of Stereotypic Behavior
The effects of CARB and AEA on APO-induced stereotypies and spontaneous grooming indicate that intercellular electrotonic coupling is involved in basal ganglia regulation of movement, and may be necessary for the expression of these basal ganglia-mediated behaviors in particular. Studies in multiple brain regions showing that electrical coupling between neurons and/or glia appears to be necessary for synchronized neural activity within these regions and the highly stereotyped behaviors mediated by such activity (Alvarez-Maubecin et al. 2000; Bou-Flores and Berger 2001; Perez Velazquez and Carlen 2000; Rothwell 1998; van den Pol and Dudek 1993). Extrapolating from these results, we propose that electrotonic coupling among basal ganglia cells may be one part of a process in which information flows across “microcircuits” in the basal ganglia, allowing behavioral switching and the completion of behavioral sequences.
Our previous data show that striatal coupling can be potently altered by corticostriatal drive acting via nitric oxide (O'Donnell and Grace 1997). We propose that, under the modulatory control of the DA system, the cortex functions to guide a sequence of motor patterns from one simple motor set to another via sequential activation and de-activation of excitation and electrical coupling among sets of functionally related neurons. The specific role of DA through D2 receptors may be to regulate the size and time course of an electrotonically-coupled network (Cepeda et al. 1989; Onn and Grace 1994). On the other hand, abnormally high D2 receptor activation would lead to persistent coupling in the most readily-activated striatal circuits (Onn and Grace 1994). This pathological neuronal process could prevent a microcircuit from “exiting out” of its sequential activation and prevent behavioral switching (Jaspers et al. 1990; Ridley 1994). Such a failure to “release” a component of a motor response would thus lead to re-entrant, repetitive activation of a simple motor component such as would occur during stereotypy when it occurs within corticostriatal motor circuits. In contrast, the selective activation of coupling within limbic circuits that occurs upon withdrawal from repeated amphetamine administration (Onn and Grace 2000) may also lead to a type of “cognitive” stereotypy, such as the perseverative drug-seeking behavior demonstrated as a consequence of drug abuse. Within this context, the present findings on the actions of gap junction inactivators may have relevance for diseases such as obsessive-compulsive disorder, drug abuse, and schizophrenia, or other disorders that are associated with disruption of cognitive and/or motor sequencing (Berger et al. 1989; Cools et al. 2001; Hymas et al. 1991; Ridley 1994; Sullivan et al. 2001).
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Acknowledgements
This work was supported by USPHS awards MH 45156, MH 57440 and MH 29670 (A.A.G.), and Young Investigator (H.M.) and Distinguished Investigator (A.A.G.) awards from the National Alliance for Research on Schizophrenia and Depression.
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Moore, H., Grace, A. A Role for Electrotonic Coupling in the Striatum in the Expression of Dopamine Receptor-mediated Stereotypies. Neuropsychopharmacol 27, 980–992 (2002). https://doi.org/10.1016/S0893-133X(02)00383-4
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DOI: https://doi.org/10.1016/S0893-133X(02)00383-4
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