Biosynthesis of Ascorbic Acid


SOME time ago1, we observed that incubation of some tissues of the rat with mannose in a mixture of Ringer-Locke solution and phosphate buffer at pH 7.4 resulted in an increase of the ascorbic acid value, as determined by the usual titrimetic technique with 2: 6-dichlorophenol indophenol2. The experiments in vitro were supported by experiments in vivo, in which it was shown that the injection of mannose into the rat also caused an increase in the ascorbic acid content of some of its tissues3. Cell-free extracts were also obtained from germinating Phaseolus mungo, which were likewise capable of converting mannose into ascorbic acid4.

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    Guha and Ghosh, NATURE, 134, 739 (1934).

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    Ghosh and Guha, J. Indian Chem. Soc., 12, 30 (1935).

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    Guha and Ghosh, NATURE, 135, 234 (1935).

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    Guha and Ghosh, NATURE, 135, 871 (1935).

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    Euler, Gartz and Malmberg, Biochem. Z., 282, 402 (1935).

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GUHA, B., GHOSH, B. Biosynthesis of Ascorbic Acid. Nature 138, 844–845 (1936).

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