Abstract
SOME years ago we showed conclusive evidence that considerable amounts of nitrogenous compounds appear in the medium in inoculated, but otherwise sterile, cultures of leguminous plants. Since then we have tried to isolate these nitrogenous compounds and to determine their chemical nature. The nitrogen compounds were extracted from the quartz sand with water and found to consist chiefly of amino acids. Amino nitrogen determinations according to van Slyke—the reaction mixture being shaken for 30 minutes—showed that 87–98 per cent of the total nitrogen was amino nitrogen. The amino nitrogen values varied to some extent in different extracts.
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References
Virtanen, v. Hausen and Karström, Biochem. Z., 258, 106; 1933.
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VIRTANEN, A., LAINE, T. Chemical Nature of the Amino Acids excreted by Leguminous Root Nodules. Nature 136, 756–757 (1935). https://doi.org/10.1038/136756b0
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DOI: https://doi.org/10.1038/136756b0
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