Letter | Published:

Biological Formation of Ascorbic Acid

Nature volume 135, page 234 (09 February 1935) | Download Citation



WE have already reported1 that the spleen, kidney and liver tissues of the rat are able to form significant amounts of ascorbic acid, as determined titrimetrically, when incubated with mannose for three hours at pH. 7·4 at 37°. The brain, heart-muscle and leg-muscle tissues of the rat have also been found to share this power, though to a less extent. It has been possible, further, to extract the mannose dehydrogenase system from the spleen, kidney and liver tissues of the rat2. A similar enzyme system has also been extracted from germinating mung (Phaseolusmungo), which can convert mannose into ascorbic acid at pH 5·8, but not at pH. 7·4. This is perhaps related to the acidity of the germinating mung.

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  1. 1.

    and , NATURE, 134, 739; 1934.

  2. 2.

    and , Current Science, 3, 251; 1934.

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  1. Biochemical Laboratory, Bengal Chemical and Pharmaceutical Works, Ltd., Calcutta. Jan. 14.

    • B. C. GUHA
    •  & A. R. GHOSH


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