Letter | Published:

Synthesis of Ascorbic Acid (Vitamin C) by means of Tissues in Vitro

Naturevolume 134page739 (1934) | Download Citation

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Abstract

The Tillmans technique of titration against the indicator 2: 6-dichlorophenol indophenol has been modified by Harris and Ray1 for the estimation of ascorbic acid in trichloroacetic acid extracts of different materials. By employing the same method, slightly modified by the introduction of glacial acetic acid before titration2, we have been for some time investigating the nature of the precursor and mechanism involved in the synthesis of ascorbic acid by the rat, a species known to be independent of an external source of the vitamin.

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References

  1. 1

    Harris and Ray, Biochem. J., 27, 303 ; 1933.

  2. 2

    Guha and Gosh, Current Science, 2, 390 ; 1934.

  3. 3

    Ray, Biochem. J., 28, 996 ; 1934.

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Affiliations

  1. Biochemical Laboratory, Bengal Chemical and Pharmaceutical Works, Ltd, Calcutta

    • B. C. GUHA
    •  & A. R. GHOSH

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https://doi.org/10.1038/134739b0

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