Letter | Published:

Synthesis of Ascorbic Acid (Vitamin C) by means of Tissues in Vitro

Naturevolume 134page739 (1934) | Download Citation



The Tillmans technique of titration against the indicator 2: 6-dichlorophenol indophenol has been modified by Harris and Ray1 for the estimation of ascorbic acid in trichloroacetic acid extracts of different materials. By employing the same method, slightly modified by the introduction of glacial acetic acid before titration2, we have been for some time investigating the nature of the precursor and mechanism involved in the synthesis of ascorbic acid by the rat, a species known to be independent of an external source of the vitamin.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Harris and Ray, Biochem. J., 27, 303 ; 1933.

  2. 2

    Guha and Gosh, Current Science, 2, 390 ; 1934.

  3. 3

    Ray, Biochem. J., 28, 996 ; 1934.

Download references

Author information


  1. Biochemical Laboratory, Bengal Chemical and Pharmaceutical Works, Ltd, Calcutta

    • B. C. GUHA
    •  & A. R. GHOSH


  1. Search for B. C. GUHA in:

  2. Search for A. R. GHOSH in:

About this article

Publication history

Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.