Staining Yeasts with Methylene Blue


    WORKERS who use the time-honoured method of staining yeasts with methylene blue are familiar with the untrustworthy results of ten obtained when an attempt is made to distinguish living from dead yeasts. It is now realised that apparently contradictory results may be due to differences in working conditions employed by various workers, and in this connexion a recent letter in NATURE (Brooks, 125, p. 599; April 19, 1930) may be cited, in which the importance of pH. value, concentration and purity of the stain, and of the effects of light are indicated.

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    Staining Yeasts with Methylene Blue. Nature 126, 491–492 (1930).

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