Figure 6 | Oncogene

Figure 6

From: Monomeric but not trimeric clathrin heavy chain regulates p53-mediated transcription

Figure 6

Cooperative effect of CHC and histone acetyltransferase p300 on p53-dependent transcription. (a) p300 interacts with CHC833-1406 as well as full-length CHC1-1675 in cells. H1299 cells were transfected with FLAG-tagged p300 and HA-tagged CHC constructs. Lysates were prepared 48 h after transfection, immunoprecipitated with anti-FLAG antibody and eluates were immunoblotted with indicated antibodies. (b) CHC833-1406 transactivates p53 in collaboration with p300. H1299 cells were transfected with 1 ng of p53 construct, 200 ng of HA-tagged p300 construct, 200 ng of CHC construct and 150 ng of reporter plasmid containing p53AIP1 promoter, and luciferase activity in cell lysates was measured 24 h after transfection. (c) A dominant-negative p300 fragment (p300DN) inhibits CHC833-1406-mediated p53 transactivation. H1299 cells were transfected with 1 ng of p53 construct, 200 ng of FLAG-tagged p300DN construct, 200 ng of CHC construct and 150 ng of reporter plasmid containing p53AIP1 promoter and luciferase activity in cell lysates was measured as described in (b).

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