Δ9-Tetrahydrocannabinol (THC) inhibits the epidermal growth factor (EGF)-induced phosphorylations of ERK1/2, JNK1/2 and AKT as well as VEGF production in NSCLC cells. A549 (a) and SW-1573 (b) NSCLC cells were incubated overnight with the indicated concentrations of THC in serum-free medium supplemented with 0.1% FBS, and then were stimulated with EGF for 10 min. The phosphorylations of ERK1/2, JNK, and AKT were analysed in the cells by western blot analysis using the indicated phospho-specific antibodies. WB, western blot. Representative blots from three stimulations are shown. VEGF production was inhibited in A549 cells (c) and SW-1573 cells (d) on treatment with different concentrations of THC for 24 h (*P<0.05; **P<0.001; compared to vehicle-treated cells).