NSCLC cell lines express cannabinoid receptors. (a) A549 and SW-1573 cell lysates were subjected to immunoblot analysis using anti-CB1, anti-CB2 or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-specific antibodies. (b) Total RNA was isolated from the cells and RT–PCR was performed using selective primers for human CB1, CB2, or GAPDH. Data shown are representative of two experiments. HMEC-1 (data not shown) and Jurkat cells were taken as positive controls for CB1 and CB2 expression.