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Identification and characterization of a novel p42.3 gene as tumor-specific and mitosis phase-dependent expression in gastric cancer

Oncogene volume 26, pages 7371–7379 (2007)Cite this article

Abstract

Multiple genetic alterations are attributed to gastric cancer (GC); however, only a few critical genes have been identified so far. In this study, we isolated and characterized a novel gene p42.3, represented as tumor-specific and mitosis phase-dependent expression protein in GC cell line BGC823. Our data showed that the expression of p42.3 was cell cycle-dependent in GC cell lines. Moreover, p42.3 was specifically expressed in primary GC tissues but not in the matched normal mucosa of stomach, and this gene was expressed in diverse embryonic tissues. Furthermore, significant suppression of cell proliferation and tumorigenicity were detected and G2/M phase arrest was observed in cell line BGC823 depleted of p42.3 expression by RNAi technique, and we confirmed the expression changes of cyclin B1 and Chk2 following the silence of p42.3. Taken together, we cloned and characterized p42.3 gene that was specifically expressed in GC tumors but not in normal gastric mucosa, and the gene was associated with M-phase regulation. Moreover, p42.3 might be involved in cell proliferation and tumorigenesis; therefore, this gene might have potential applications in the diagnosis or treatment of GC.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (39625016), State Key Basic Research Program of China (2004CB518708) and the National High Technology Research and Development Program of China (2002BA711A11).

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Author notes

  1. X Xu and W Li: These authors contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Molecular Oncology, Beijing Institute for Cancer Research, School of Oncology, Peking University, Hai-Dian District, Beijing, China,

    X Xu, W Li, X Fan, Y Liang, M Zhao, J Zhang, Y Liang & Y Lu

  2. Beijing Genomics Institute, Chinese Academy of Sciences, Beijing Airport Industrial Zone, Shunyi District, Beijing, China,

    W Tong & J Wang

  3. National Engineering Research Center for Beijing Biochip Technology and Tsinghua University School of Medicine, Changping District, Beijing, China,

    W Yang

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  1. X Xu
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  2. W Li
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Corresponding author

Correspondence to Y Lu.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Xu, X., Li, W., Fan, X. et al. Identification and characterization of a novel p42.3 gene as tumor-specific and mitosis phase-dependent expression in gastric cancer. Oncogene 26, 7371–7379 (2007). https://doi.org/10.1038/sj.onc.1210538

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