Table 2: The effect of E1A12S deletion mutants on EGFR expression in HN5 cells

From: p400 function is required for the adenovirus E1A-mediated suppression of EGFR and tumour cell killing

ConstructaLocalizationEGFR suppressionResidues from E1A12S
dl1101-GFP (Δ569–634)NuclearNoDeletion aa 4–25, does not bind p300 or p400
dl1102-GFP (Δ635–664)NuclearNoDeletion aa 25–35, does not bind p400 but still binds p300
dl1103-GFP (Δ647–706)NuclearNoDeletion aa 30–49, does not bind p400 or p300
dl1104-GFP (Δ701–739)NuclearYesDeletion aa 48–60, does not bind p300
dl1107-GFP (Δ890–928)NuclearYesDeletion aa 111–123, does not bind pRB
dl1102/06 PartiallyDeletion aa 26–35 and 90–105, does not bind p400 but still binds p300
dl1102/08 PartiallyDeletion aa 26–35 and 124–127, does not bind p400 or pRB, but still binds p300
dl1105/07 YesDeletion aa 70–81 and 111–123, does not bind pRB
1–158-GFPCytoplasmic/nuclearYesDeletion aa 159–243, binds pRB, p300, p400
RG2-GFPNuclearYesPoint mutation at 2 from R to G, does not bind p300, reduced binding to pRB
Y47H-GFPNuclearYesPoint mutation at 47 from Y to H, does not bind pRB or p130 in HeLa cells, but does bind pRB in BRK cells (Wang et al., 1993)
Y47H/C124G-GFPNuclearNoPoint mutations at 47 from Y to H and at 124 C to G, double mutant, does not bind pRB and p107 in HeLa or in BRK cells (Wang et al., 1993).
  1. Abbreviations: EGFR, epidermal growth factor receptor; GFP, green fluorescent protein.
  2. aThe position of base-pair deletions in the E1A12S is shown in bracket.