Figure 5

From: p400 function is required for the adenovirus E1A-mediated suppression of EGFR and tumour cell killing

Figure 5

(a) MTT analysis of HCT116 and HCT116 p300−/− cells 48 h after infection with Ad-E1A12S or control Ad-Del adenoviral vectors at a MOI of 10. Error bars indicate s.d. (b) Western blot analysis of HCT116 and HCT116 p300−/− cells infected with indicated recombinant adenoviruses. Cells were lysed 24 h after infection, equal amounts of total protein from each sample was separated by SDS–PAGE, transferred to nitrocellulose and hybridized to different antibodies as described in the Materials and methods section. Abbreviations: MOI, multiplicity of infection; MTT, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide; SDS–PAGE, lauryl sulphate–polyacrylamide gel electrophoresis.