Figure 2

From: p400 function is required for the adenovirus E1A-mediated suppression of EGFR and tumour cell killing

Figure 2

Downregulation of EGFR expression by the 12S-encoded E1A proteins of human adenovirus types 2, 3, 9 and 12, but not 4 and 40. HN5 cells were transiently transfected with GFP plasmids expressing E1A12S of different human adenovirus types. Cells were fixed 48 h post-transfection and stained for EGFR by indirect immunofluorescent staining using anti-EGFR F4 primary antibody and Texas-Red-labelled secondary anti-mouse antibody. Cells were mounted in DAPI-containing solution. EGFR stained red and E1A-GFP was observed as green. E1A expressing cells are indicated with white arrows. Abbreviations: DAPI, 4′,6-diamino-2-phenylindole; EGFR, epidermal growth factor receptor; GFP, green fluorescent protein.