Figure 1

From: p400 function is required for the adenovirus E1A-mediated suppression of EGFR and tumour cell killing

Figure 1

(a) Different splice variants of the E1A gene are schematically depicted. Boxes represent coding sequences. The coding sequence represented by the light grey box in E1A9S is in a different frame from the other coding sequences. The number of amino acids of the encoded E1A isoforms and the positions of the conserved regions CR1, CR2, CR3 and CR4 in the different E1A splice variants are indicated (CR positions have previously been described in Avvakumov et al., 2002, 2004). (b) Downregulation of EGFR expression by adenovirus type 2 E1A12S. HN5 cells were transiently transfected with plasmids expressing adenovirus type 2 E1A9S, E1A10S, E1A11S, E1A12S and E1A13S fused to GFP. Cells were fixed 48 h post-transfection and stained for EGFR by indirect immunofluorescent staining using anti-EGFR F4 primary antibody and Texas-Red-labelled secondary anti-mouse antibody. Cells were mounted in DAPI-containing solution. EGFR stained red and E1A-GFP was observed as green. E1A expressing cells are indicated with white arrows. Abbreviations: CR, conserved region; DAPI, 4′,6-diamino-2-phenylindole; EGFR, epidermal growth factor receptor; GFP, green fluorescent protein.