Figure 4 | Oncogene

Figure 4

From: Epigenetic regulation (DNA methylation, histone modifications) of the 11p15 mucin genes (MUC2, MUC5AC, MUC5B, MUC6) in epithelial cancer cells

Figure 4

Histone modification status at MUC2 and MUC5B promoters in PANC-1, LS174T, HT-29 STD and HT-29 5F7 cancer cells. Chromatin immunoprecipitation (ChIP) assays were performed as described in Materials and Methods. Acetylated histone H4 (AcH4) and six histone H3 modifications were analysed: acetylated lysine (AcH3), acetylated lysine 9 (Ac-K9H3), acetylated lysines 9 and 14 (Ac-K9/14H3), monomethylated lysine 9 (Me-K9H3), mono/di/trimethylated lysine 4 (m/d/tMe-K4H3) and trimethylated lysine 27 (tMe-K27H3). Input DNA was used as a positive amplification control; IgG indicates ChIP performed using rabbit IgGs as an isotypic antibody control.

Back to article page