Table 2 Microsatellite analysis of chromosome arm 17q in five leukemic samples from patients with JMML

From: Genome-wide single-nucleotide polymorphism analysis in juvenile myelomonocytic leukemia identifies uniparental disomy surrounding the NF1 locus in cases associated with neurofibromatosis but not in cases with mutant RAS or PTPN11

Marker Genomic position (Mbp) Frequency of heterozygotes D003 (NF-1) D102 (NF-1) D115 (NF-1) D119 D126 (NF-1)
D17S925 24.33 0.72 151 153 153 153 153
     161   155  
D17S1800 26.96a 0.74 270 276 278 268 276
       280  
D17S1880 28.03 0.83 182 170 186 180 188
       186  
D17S855 38.45 0.82 143 147 143 145 149
       149  
D17S1827 43.99 0.83 140 144 142 136 134
       140  
D17S787 50.63 0.80 150 138 136 138 136
       150  
D17S948 58.34 0.83 141 141 127 127 127
D17S784 74.42 0.77 234 232 230 228 230
       232  
  1. Abbreviation: NF-1, clinical diagnosis of neurofibromatosis type 1.
  2. Primer information for UniSTS markers can be found at http://www.ncbi.nlm.gov/genome. Each locus was PCR-amplified using a fluorescently labeled forward primer (Sigma-Proligo, The Woodlands, TX, USA). Length polymorphisms of PCR products were detected by capillary electrophoresis (CEQ2000XL, Beckman Coulter, High Wycombe, UK). The numbers in each sample column indicate microsatellite fragment lengths in base pairs as provided by the analysis software. Heterozygote frequencies of polymorphic markers were extracted from the GDB human genome database at http://www.gdb.org.
  3. aGenomic position of the NF1 gene on chromosome 17 is 26.66 Mbp.