Schematic map of exon 1–5 region and 138-bp CpG island in FAT gene. Exons are indicated by an open box, with each size shown in parentheses. Arrows at the bottom show the positions of primers for PCR, RT-PCR and MSP. Probes for Southern blot are indicated by a vertical box and the CpG island is indicated by a hatched box. Primer sequences are indicated in Table 2.