Some of the ChIP-chip ARBSs associate with histone acetylation and RNA PolII recruitment. Hormone-deprivated LNCaP cells were stimulated with R1881 (10 nM) or vehicle for 24 h. Cross-linked samples were immunoprecipitated with anti-acetylated H3/H4 (AcH3/AcH4) or anti-RNA PolII antibodies. The precipitated DNA fragments were subjected to qPCR. Identical primer sets were used as described in Figure 2. Data are fold enrichment compared to individual input non-enriched DNA (mean±s.d., n=2).