Androgen-dependent changes in expression of genes adjacent to the ChIP-chip ARBSs in LNCaP cells. Hormone-deprivated cells were stimulated with R1881 (10 nM) or vehicle for 12, 24 and 48 h. Quantitative RT–PCR analysis regarding the expression of 10 proximal genes close to ARBSs no. 1–no. 10 was performed using the reverse-transcribed cDNAs from the cells. Data are fold change compared with vehicle-treated cells at individual time point (mean±s.d., n=2). (a) UGT1A1 mRNA levels, (b) PGC mRNA levels and (c) mRNA levels of gene adjacent to ARBSs no. 3–no. 10.