ISH-TMA analysis of representative genes. Left: ISH images of the epithelial-associated (CLDN18 and TFF2) and stromal (SULF1, CDH11 and COL8A1) genes on EGC and AGC. The bright field panels (BF) show hematoxylin/eosin counterstaining; the dark field panels (DF) show the ISH signals (bright areas) at × 20 magnification. The boxed area is also shown at higher magnification (HM panels, only the bright field is shown), with indication of the tumor (T) and stromal (S) areas. Right: Bar graph representation of the percentage of samples in which a positive signal (ISH scores 2–3) was observed in normal mucosa, EGC and AGC. Asterisks indicate the statistical significance (P<0.05) of differential expression in EGC and AGC tumor classes vs normal samples (Fisher's exact test). Actual numbers and statistical analysis are in Supplementary Table 4.