Disruption of Scribble in the rumz mouse results in defective embryonic wound healing in vivo. (a) Schematic representation of the position of the rumpelstilzchen (rumz) mutation (I285K) in the Scribble protein. (b) Western blot of whole-cell lysates from basal keratinocytes isolated from the skin of E17.5 embryos, derived from scribble+/rumz heterozygous crosses and cultured ex vivo for 4 days. Quantitation of Scribble protein level, relative to wild-type, is shown below. (c) E12.5 embryos wounded by surgical removal of the left lower limb, cultured ex vivo for 20 h and visualized by scanning electron microscopy.