Scribble knockdown causes defective chemotactic migration. 50 000 cells (EGF starved) were plated in the upper well of a transwell chamber in media containing no EGF. Cells were allowed to migrate towards EGF containing media in the lower chamber over 16 h. Two fields (× 10 objective) of DAPI stained, migrated cells were counted for each condition performed in duplicate. The difference in cell number between Scribble knockdown and shEGFP control cells was statistically significant (P<0.05, n=3, Student's t-test). Error bars represent s.d. of the mean.