Scribble knockdown causes defective wound closure in vitro. (a) Confluent MCF10A monolayers were starved of EGF overnight, wounded with a pipette tip and allowed to migrate for 24 h in the presence of 20 ng/ml EGF and 1 ng/ml Mitomycin C (to block cell proliferation). (b) Quantitation of the movement of the migrating cell front 24 h after wounding normalized relative to MCF10A parental cell movement. The decrease in migration of each Scribble knockdown population was statistically significant when compared to shEGFP control cells (P<0.01, n=3, Student's t-test). Error bars represent s.d. of the mean.