Table 1b Large section FISH analysis for E2F3 and NM_017774

From: E2F3 is the main target gene of the 6p22 amplicon with high specificity for human bladder cancer

  Sample Stage/grade Gene copy number Ratio Final score
    C6 E2F3 NM_017774 E2F3 NM_017774  
 1 PT16 pT1/G3 2–3 2–3 2–3 1.0 1.0 Normal
 2 PT17 pT1/G3 2–4 2–4 2–4 1.0 1.0 Normal
 3 PT18 pT1/G3 2–4 2–4 2–4 1.0 1.0 Normal
 4 PT19 pT2–/G3 2–5 2–8 2–6 1.4 1.1 Gain
 5 PT20 pT1/G3 4–8 4–12 4–12 1.3 1.3 Gain
 6 PT21 pT2–/G3 2–6 2–10 2–6 1.5 1.5 Gain
 7 PT22 pTa/G3 2–10 10–20 10–20 2.5 2.5 Gain
 8 PT23 pT1/G3 2–4 6–10 6–10 2.7 2.7 Gain
 9 PT24 pT2–/G3 2–4 10 10 3.3 3.3 Co-amplified
10 PT25 pT2–/G3 4 10–20 20 3.8 5.0 Co-amplified
11 PT26 pT2–/G3 2 10 10 5.0 5.0 Co-amplified
12 PT27 pT1/G3 2–4 10–20 10–20 5.0 5.0 Co-amplified
13 PT28 pT2–/G3 2–4 10–20 10–20 5.0 5.0 Co-amplified
14 PT29 pT1/G3 2–4 10–30 10–30 6.7 6.7 Co-amplified
15 PT30 pT1/G3 1–2 10–20 10–20 10.0 10.0 Co-amplified
16 PT31 pT1/G3 2–4 30 30 10.0 10.0 Co-amplified
17 PT32 pT2–/G3 2–6 50 50 16.7 16.7 Co-amplified
18 PT33 pT2–/G3 Tissuea
19 PT34 pT1/G3 Tissueb
  1. Abbreviations: FISH, fluorescence in situ hybridization; PT, primary tumour.
  2. aTissue=insufficient tissue left.
  3. bTissue=tissue damage.