Figure 3 | Oncogene

Figure 3

From: Transcriptional activation of cyclooxygenase-2 by tumor suppressor p53 requires nuclear factor-kappaB

Figure 3

Effect of mutant p53 on cyclooxygenase-2 (Cox-2) expression. (a) Induction of Cox-2 expression by mutant p53. HCT116 cells were co-transfected with increasing amounts of wild-type (WT) or mutant p53 expression vectors. Top, COX-2 mRNA was quantitated by real-time PCR using GAPDH as internal control. Bottom, cell extracts were analysed by Western blot with anti-Cox-2 antibody using β-actin antibody as loading control. (b) Wild-type p53-dependent effect of mutant p53 on COX-2 promoter activation. HCT116 (upper panel) or HCT116-p53−/− cells were co-transfected with COX-2-LUC reporter, control (Ctrl) or increasing amounts of WT or mutant p53 expression vectors (0.1, 0.3, 1 μg), and RSV-βGAL as internal transfection control. LUC activity was expressed as fold induction normalized to βGal.