Table 3 Immunochemistry staining of POU2F3 in cervical tissues

From: Aberrant promoter methylation and silencing of the POU2F3 gene in cervical cancer

Type of epithelium Evaluated cases Cytoplasm staining Number of cases Percent cellular staining** (%) Comments
   No staining Intensity 1 Intensity 2–3*   
Normal cervix 13 0 1 12 70–100 No staining epithelial membrane; stroma or basal cells
Koilocytotic atypia and CIN 1 (low grade) Squamous 0 0 7 70–100 100% of reserve cells stained (four cases)
  11   0 1 50–70  
    0 3 20–50  
  Glandular 0 2 0 20–50  
  6   0 4 5–20  
CIN 2 and 3 (high grade) Squamous 0 6 3 70–100  
  21   3 4 50–70  
    2 1 20–50  
    0 2 5–20  
  Glandular 0 2 1 20–40  
  3      
Squamous carcinoma*** 9 4 2 0 80–90  
    3 0 <30  
Adenocarcinoma 10 2 1 1 100  
    2 2 50–70  
     2 10–25  
  1. CIN=cervical intraepithelial neoplasia. *P<0.01, staining intensity decreased as the CIN grade increased compared to normal cervix. **P<0.01, percentage of cellular staining in CIN is significantly decreased compared to normal cervix. ***P<0.01 overall, loss of POU2F3 in squamous carcinoma is significant compared to normal cervix.