Figure 4 | Oncogene

Figure 4

From: TIPUH1 encodes a novel KRAB zinc-finger protein highly expressed in human hepatocellular carcinomas

Figure 4

Interaction of transcription-involved protein upregulated in HCC (TIPUH1) with hnRNPU, TIF1β, nucleolin or hnRNPF. HEK293 cells were transiently transfected with p3xFLAG-TIPUH1, or p3xFLAG empty vector (mock). Lysates were immunoprecipitated with murine anti-FLAG M2 antibody conjugated with agarose beads and specific antibodies to interacting proteins. (a and b) Co-immunoprecipitation of FLAG-TIPUH1 and endogenous hnRNPU. Whole-cell extracts from cells exogenously expressing FLAG-TIPUH1 were used as positive controls. (c and d) Co-immunoprecipitation of FLAG-TIPUH1 and endogenous nucleolin. (e) Co-immunoprecipitation of FLAG-TIPUH1 and endogenous TIF1β. (f) Co-immunoprecipitation of FLAG-TIPUH1 and endogenous hnRNPF.

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