Subcellular localization of transcription-involved protein upregulated in HCC (TIPUH1). (a) Western blot analysis of exogenously expressed FLAG-tagged TIPUH1 protein in HEK293 cells. Empty p3xFLAG-CMV10 (mock) vector served as a negative control. (b) FLAG-tagged TIPUH1 protein was expressed exogenously into SNU475 (HCC) and HepG2 hepatoma cells, and stained with anti-FLAG monoclonal antibody. The protein was visualized by rhodamine-conjugated secondary anti-mouse IgG antibody or FITC-conjugated secondary anti-mouse IgG antibody (left panel). Nuclei were counterstained with 4′,6′-diamidine-2′-phenylindole dihydrochloride (DAPI) (middle panel); merged images (Rhodamine or FITC and DAPI) are shown in the right panel. Magnifications × 400 (SNU475); × 600 (HepG2).